Multienzyme Synthesis of Glycyrrhetic Acid 3-O-mono-β-d-glucuronide by Coupling UGT73F15 to UDP-Glucuronic Acid Regeneration Module

Glycyrrhetic acid 3-O-mono-β-d-glucuronide (GAMG), a rare and innovative compound in licorice, exhibits high-potency sweetness improved physiological activities. However, low amounts of GAMG from plants cannot meet the demands growing markets. In this study, an efficient one-pot multienzyme cascade reaction for biosynthesis was constructed using coupled catalysis glycosyltransferase uridine 5′-diphosphate (UDP) glucuronic (GlcA) regeneration system. The Glycyrrhiza uralensis UGT73F15 expressed Escherichia coli BL21 (DE3). optimal conditions were found to be pH 7.5 35 °C. catalytic efficiency (kcat/Km) glycyrrhetic (GA) 2.14 min−1 mM−1 when UDP-GlcA as sugar donor. To regenerate costly UDP-GlcA, including UGT73F15, sucrose synthase, UDP-glucose dehydrogenase, lactate dehydrogenase adopted synthesize GA on basis By optimizing conditions, production successfully achieved 226.38 mg/L. Our study developed economical method facile synthesis other bioactive glucuronosides.